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. 2017 Nov 2;7:14932. doi: 10.1038/s41598-017-06500-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GP73 Activates SREBPs via Upregulation of SCAP. (a) Immunoblotting analysis of SCAP protein in HepG2 or HL7702 cells transfected with scrambled siRNA or SCAP-specific siRNA duplexes. α-Tubulin was used as equal loading control. (b) Immunoblotting analysis of SREBP1 activation in Flag-vector or Flag-SCAP HepG2 cells transfected with Myc-vector or Myc-GP73. α-Tubulin was used as equal loading control. (c) Immunoblotting analysis of SREBP1 activation in siSCAP knockdown or control HepG2 cells transfected with Flag-vector or Flag-GP73. α-Tubulin was used as equal loading control. (d,e) SREBP-1 promoter activity in siSCAP knockdown or control HL7702 cells and HepG2 cells transfected with Flag-vector or Flag-GP73. The luciferase activity was measured 36 hrs post transfection. Data was normalized based on transfection efficiency. Cell-based studies were performed at least three independent times with comparable results. Numbers below certain Western blots indicate relative levels determined by software-based quantification of the representative experiment shown. Data represent mean ± SEM. Student’s t test was used for statistical analysis: **p < 0.01.