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. 2017 Nov 2;7:14897. doi: 10.1038/s41598-017-15006-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the expression levels of the GABA-synthesizing enzyme isoform GAD67 in young adult and old rats. The top panel shows typical GAD67 western blotting results for young rats (YR1, YR2, YR3) and old rats (OR1, OR2, OR3) using GAD67 and GAPDH antibodies (see also the full-length blots presented in Supplementary Figure 2). The bottom panel shows the mean optical density (OD) of the GAD67 band normalized to the OD of the band corresponding to GAPDH in the two groups of rats. The mean normalized OD value of the GAD67 band in old rats (0.694 ± 0.075) was significantly lower than that in young rats (0.872 ± 0.079) (two-tailed t-test, p = 0.0027).