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. 2017 Nov 2;7:14897. doi: 10.1038/s41598-017-15006-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of the expression levels of the GABA_A receptor subunit α₁ (GABA_AR α₁) in young adult and old rats. The top panel shows typical western blotting results obtained for young rats (YR1, YR2, YR3) and old rats (OR1, OR2, OR3) using GABA_AR α₁ and GAPDH antibodies (see also the full-length blots presented in Supplementary Figure 3). The bottom panel shows the mean optical density (OD) of the band corresponding to GABA_AR α₁ normalized to the OD of the band corresponding to GAPDH in the two age groups. The mean normalized OD value of GABA_AR α₁ in old rats (0.308 ± 0.127) was significantly smaller than that in young rats (0.513 ± 0.099) (two-tailed t-test, p = 0.011).