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. 2017 Nov 2;7:14888. doi: 10.1038/s41598-017-13993-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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B cells expressing higher levels of BCR have elevated viability and tonic signal than B cells expressing low levels of BCR. (A) B cells were gated into 10 population based on their BCR levels. Each population contained the same numbers of B cells. (B) Viability of each gated B cell population before culture (0 h) and at 18 h, 48 h and 72 h after culture in medium alone. Levels of phosphorylated ERK (pERK) (C and D) and pAKT (E and F) in gated BCR^low and BCR^high population are shown. Representative FACS profiles (C and E) and mean ± SD of 3 independent experiments (D and F) are shown. Gating strategies are outlined in Supplementary Figure S2. *p < 0.05 (paired t test).