Table 2.

Mixed linear regression model estimates for ACTH concentrations (ng/ml) measured in diluted medium samples from cells transfected with KCNQ1

Parameter	Estimate	SE	p
Intercept (WT KCNQ1)a	7.26	0.99	<0.001
Time	0.76	0.25	0.004
KCNQ1-Arg116Leu	0.32	0.72	0.659
KCNQ1-Pro369Leu	0.62	0.72	0.393
KCNQ1-Gly589Asp	1.11	0.72	0.126

Mixed linear regression model estimates for ACTH concentrations (ng/ml) measured in diluted medium samples (1:10) in the reference condition (WT KCNQ1) and three other KCNQ1 conditions (KCNQ1-Arg116Leu, KCNQ1-Pro369Leu, KCNQ1-Gly589Asp), their standard errors (SE), and corresponding P values. The model included both the growth time (per 1 h) and the KCNQ1 conditions as fixed effects and the experiment number as a random intercept. ACTH levels produced by the cells transfected with mutant KCNQ1s did not differ from the levels produced by the WT KCNQ1. Similarly, the CRF-induced ACTH secretion did not differ between the four KCNQ1 environments (data not shown)

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