Fig. 4.

DNA sequencing of pair-end reads starting at the ends of LTRs of Onsen reveals recombinant LTRs in ecDNA of heat-stressed wild-type and nrpd1-3 plants. a Non-discriminatory pair-end reads (see text). b Discriminatory pair-end reads reveal the occurrence of recombinant LTRs. Colour code as in Fig. 1a. Horizontal or vertical bars are present only in those cases where NGS reads matching old Onsen members contained polymorphisms that allowed discrimination of 5′ and 3′ LTRs