Skip to main content
. 2017 Aug 26;8(6):613–619. doi: 10.1111/1759-7714.12492

Figure 4.

Figure 4

PDCD 4 overexpression restrains proliferation and invasion induced by miR‐155. After co‐transfection with miR‐155 NC or miR‐155 and pcDNA3.1‐PDCD 4, the PDCD 4 protein level was analyzed by Western blot in (a) A549 and (b) H2170 cells. β‐actin was used as an internal control. Cell growth activity after co‐transfection with miR‐155 NC or miR‐155 and pcDNA3.1‐PDCD 4 was assessed using CCK‐8 assays in (c) A549 and (d) H2170 cells (*P < 0.05). (e) Six weeks after injecting the mice with miR‐155 NC, miR‐155 NC + pcDNA3.1‐PDCD 4, miR‐155, or miR‐155 + pcDNA3.1‐PDCD 4 group cells (2 × 106 A549 or H2170 cells), the nude mice were all sacrificed to compare the tumor volume in the four different groups (*P < 0.05). The average numbers of (f) A549 and (g) H2170 cells penetrating the transwell membrane were analyzed using transwell invasion assay (*P < 0.05).