Fig. 4.

Up-regulation of FABP4 in the testis of L110R MM-1α mice. (A-a) and (A-b) Total RNAs and proteins were extracted from testes of wild-type and L110R MM-1α mice, and RT-PCR (a) and Western blotting with anti-FABP4 and anti-GAPDH antibodies (b) were carried out as described in Section 2. Relative expression of fabp4 versus gapdh is shown in (a). Values are means±S.E. n=8 experiments. Significance: *p<0.05. (A-c) Testes were isolated from wild-type and L110R MM-1α mice and tissue sections were prepared. Tissue sections were then reacted with an anti-FABP4 antibody followed by HE staining, and their images were obtained as described in the legend for Fig. 2B. Two figures of the same section with different magnification are shown. (B) Total RNAs and proteins were extracted from cell lines derived from wild-type and L110R and MM-1α mice and subjected to real-time PCR and Western blotting with anti-FABP4, anti-MM-1 and anti-GAPDH antibodies as described in Section 2.