Table 1.
Nucleotide sequences of the upper and lower strands and PCR conditions for real-time PCR and RT-PCR
| Mouse gene | Sense/anti-sense | Nucleotide sequence | PCR condition |
|---|---|---|---|
| GAPDH | Sense | 5′-AACCTGGCATTGTGGAAGG-3′ | 95 °C 2 min, 95 °C 30 s, 55 °C 30 s, 33 cycles of 72 °C 1 min |
| Anti-sense | 5′-ACACATTGGGGGTAGGAACA-3′ | ||
| FABP4 | Sense | 5′-ATGCCTTGTGGGAACCTGGAAGC-3′ | 95 °C 2 min, 95 °C 30 s, 55 °C 30 s, 33 cycles of 72 °C 1 min |
| Anti-sense | 5′-GGCCATGCCACTTTCCTT-3′ | ||
| MM-1 | Sense | 5′-GGGAATTCATGGCGCAGTCGATT-3′ | 95 °C 2 min, 95 °C 30 s, 55 °C 30 s, 35 cycles of 72 °C 1 min |
| Anti-sense | 5′-GGCTCGAGTCAGGCTTTGACCGT-3′ | ||
| PLa2g3 | Sense | 5′-GGCTGAGGCCACCTCATATACTTC-3′ | 95 °C 2 min, 95 °C 30 s, 55 °C 30 s, 33 cycles of 72 °C 1 min |
| Anti-sense | 5′-TCCTTTGCCCTCAGCACAGTCAAG-3′ | ||
| PLa2g10 | Sense | 5′-GGATTGTGTTGGGCCTCGAT-3′ | 95 °C 2 min, 95 °C 30 s, 55 °C 30 s, 33 cycles of 72 °C 1 min |
| Anti-sense | 5′-TGGTAGCAGCACCAGTCAAT-3′ | ||
| Speer-4d | Sense | 5′-GCCTTCCACCTGAGAGTAATGAAGGA-3′ | 95 °C 2 min, 95 °C 30 s, 55 °C 30 s, 33 cycles of 72 °C 1 min |
| Anti-sense | 5′-AATCAGCCATGGCTTCCTAGTAAAGG-3′ |