Fig. 2.

Tryptophan fluorescence spectra of LF in the absence and presence of 6 M GdnHCl. LF (0.5 µM) was incubated in the absence (solid line), and presence of 6 M GdnHCl (dashed line) for 24 h at room temperature prior to recording emission spectra. The excitation wavelength was set to 295 nm to allow for the selective excitation of LF׳s tryptophan residues.