Fig. 2.
Effects of PGA2, PGE2, PGD2, PGF2α, PGJ2, and PGI2 on EC permeability caused by low dose of thrombin. A: pulmonary EC were treated with indicated PG (0.5 µM, first arrow) for 15 min followed by addition of low dose of thrombin (Thr; 0.2 U/ml, second arrow). Changes in TER were monitored over 2-h time period. B: analysis of EC permeability for macromolecules using XPert assay. Bar graph depicts permeability changes after 5 min of thrombin treatment; n = 4. *P < 0.05 vs. thrombin. C: cells treated with indicated PG (0.5 µM, 15 min) or vehicle were stimulated with thrombin for 30 min, and the levels of MLC phosphorylation were evaluated by Western blot with diphospho-MLC antibody. Probing with β-actin antibody was used as a normalization control. Results of densitometry are shown as means ± SD; n = 3. *P < 0.05.