Fig. 3.
Effects of PGA2, PGE2, PGD2, PGF2α, PGJ2, and PGI2 on EC permeability caused by high dose of thrombin. A: pulmonary EC were treated with indicated PG (0.5 µM, first arrow) for 15 min followed by addition of thrombin (0.5 U/ml, second arrow). Changes in TER were monitored over 3-h time period. B: analysis of EC permeability for macromolecules using XPert assay. Bar graph depicts permeability changes after 5 min of thrombin treatment; n = 5. *P < 0.05 vs. thrombin. C: visualization of agonist-induced permeability in endothelial monolayers. HPAEC monolayers grown on coverslips coated with biotinylated gelatin were treated with corresponding PG (0.5 µM, 15 min) or vehicle followed by stimulation with thrombin (0.5 U/ml, 5 min) and addition of FITC-avidin tracer as described materials and methods. Green fluorescence depicts areas permeable for FITC-labeled avidin; bar = 20 µm.