Table 1.

Primers used to amplify EP receptors and renin 1c gene

	Forward	Fluorogenic Probe	Reverse	Size, bp
EP1	5′-CTACCCACACGGCCTTAAAA-3′	5′6-FAM-GCATGTTCCCATCTTGACCT-BHQ1-3′	5′-TCTGGACTGGAGTGTGCTTG-3′	156
EP2	5′-GGTGGTGCTGGCTTCATATT-3′	5′6-FAM-CCTTCTTTAGTCTGGCCACG-BHQ1-3′	5′-CAGGGAACAGAAGAGCAAGG-3′	250
EP3	5′-TACCTGTTTCCCTGGGTCTG-3′	5′6-FAM-CAAAGGTTCTGAGGCTGGAG-BHQ1-3′	5′-TTCTTCTCCCAGGGAAGGAT-3′	263
EP4	5′-CCATCGCCACATACATGAAG-3′	5′6-FAM-CCACGCCTACTTCTACAGCC-BHQ1-3′	5′-TGCATAGATGGCGAAGAGTG-3′	209
Renin 1	5′-AGTACTATGGCGAGATCGGCATT-3′	5′6-FAM-TTCAAAGTCATCTTTGACACGGGTTCAG-BHQ1-3	5′-TGATCCGTAGTGGATGGTGA-3′	200
β-Actin	5′-ATCATGAAGTGTGACGTTGA-3′	5′6-HEX-TCTATGCCAACACAGTGCTGTCTGGT-BHQ2-3′	5′-GATCTTCATGGTGCTAGGAGC-3′	138

qRT-PCR was normalized by using the β-actin housekeeping gene.