Fig. 7.

Adenylyl cyclase 6 (AC-6), but not adenylyl cyclase 3 (AC-3), expression is higher in PN cells than in PH cells and is reduced by ACY-1215. A: Western blot showing the expression of AC-6 and AC-3 in 10 µM ACY-1215-treated or control cells. B: expression of AC-6 is much higher in PN than in PH cells and is reduced by ACY-1215 treatment. C: AC-3 is expressed to an equal extent in PN and PH cells and is not significantly affected by ACY-1215. Bars represent averages ± SE of the AC-6 and -3 expression. Data were analyzed by nonparametric t-test. The experiment was repeated 3 times. ****P < 0.0001. Note that the band intensity has been increased for PN cells for viewer convenience, since AC-6 is expressed much more abundantly in PN cells than in PH cells.