Fig. 1.

Protein kinase N2 (PKN2) knockdown decreases insulin responsiveness of glucose metabolism in skeletal muscle. A and B: mRNA levels of PKN2, PAX7, MYOG (myogenin), and DES (desmin) (A) and protein abundance of PKN2, PAX7, and DES (B) in small-interfering (si)RNA-treated primary HSMCs. C: representative bright-field images of siRNA-treated primary human skeletal muscle cells (HSMC). Scale bar, 100 µm. D–F: basal and insulin-stimulated (120 nM) glucose uptake (D), incorporation into glycogen (E), and oxidation (F) in siRNA-treated primary HSMC. Open bars, scrambled (SCR); black bars, small-interfering (si)PKN2. *PKN2 effect, P < 0.05; #insulin main effect, P < 0.05. Results are means ± SE for n = 5 biological replicates.