Fig. 5.

PKN2 knockdown decreases mammalian target of rapamycin (mTOR) signaling and protein synthesis. A:Western blot analysis of p-mTOR Ser²⁴⁴⁸, mTOR, p-S6 Ser^235/236, and S6 in primary HSMCs incubated in the absence or presence of insulin (120 nM; 15 min) (representative immunoblot from n = 5 biological replicates). B and C: quantification of p-mTOR Ser²⁴⁴⁸ (B) and p-S6 Ser^235/236 abundance (C). D: protein synthesis in siRNA-treated primary HSMCs. Open bars, SCR; black bars, siPKN2. *PKN2 effect, P < 0.05; #insulin main effect, P < 0.05. Results are means ± SE for n = 5 biological replicates.