Table 2.
Primers used
| Forward | Reverse | |
|---|---|---|
| Human | ||
| rplp0 | TGGAGAAACTGCTGCCTCAT | GATTTCAATGGTGCCCCTGG |
| ppia | AGGGTTCCTGCTTTCACAGA | CAGGACCCGTATGCTTTAGG |
| pkn2 | ATTGTGGCTCGAGATGAAGT | TTTGGTTTGGAAACATGCAA |
| pax7 | GAGGACCAAGCTGACAGAGG | CTGGCAGAAGGTGGTTGAA |
| myog | GCTCAGCTCCCTCAACCA | GCTGTGAGAGCTGCATTCG |
| des | CTGGAGCGCAGAATTGAATC | GGCAGTGAGGTCTGGCTTAG |
| ppara | TTCGCAATCCATCGGCGAG | CCACAGGATAAGTCACCGAGG |
| ppard | CAGGGCTGACTGCAAACGA | CTGCCACAATGTCTCGATGTC |
| pgc1a | TCTGAGTCTGTATGGAGTGACAT | CCAAGTCGTTCACATCTAGTTCA |
| cpt1b | CATGTATCGCCGTAAACTGGAC | TGGTAGGAGCACATAGGCACT |
| fabp3 | TGGAGTTCGATGAGACAACAGC | CTCTTGCCCGTCCCATTTCTG |
| pdk4 | GGAAGCATTGATCCTAACTGTGA | GGTGAGAAGGAACATACACGATG |
| srebp1c | GTTGGCCCTACCCCTCC | CTTCAGCGAGGCGGCTT |
| fasn | CCACAACTCCAAGGACACAG | CTGCTCCACGAACTCAAACA |
| scd1 | CCTGCGGATCTTCCTTATCA | GCCCATTCGTACACGTCATT |
| acc2 | CTGAGAGTGCGGAGGACTTC | AGCGAGGATCTGAACTTCCA |
| dgat1 | GTCCCTCTGCGAATGTTCC | GCTATTGGCTGTCCGATGAT |
| gpat1 | AACACCAGATGGACGGAAAG | CCGAGCACAAGAGGTTTTTC |
| Mouse | ||
| pkn2 | CGACCAAAACTCCAAAGACA | GTCTTCCCCAAGTGGCAATA |
| 36b4 | CCCTGAAGTGCTCGACATCA | TGCGGACACCCTCCAGAA |
myog, Myogenin; rplp0, ribosomal protein lateral stalk subunit P0; ppia, peptidylpropyl isomerase A; des, desmin; ppara and -d, peroxisome proliferator-activated receptor-α and -δ, respectively; pgc1a, PPAR coactivator-1α; cpt1b, carnitine palmitoyltransferase-1β; fabp3, fatty acid-binding protein 3; pdk4, pyruvate dehydrogenase kinase 4; srebp1c, sterol regulatory element-binding protein-1c; fasn, fatty acid synthase; scd1, stearoyl-CoA desaturase-1; acc2, acetyl-CoA carboxylase 2; dgat1, diacylglycerol O-acyltransferase 1; gpat1, glycerol-3-phosphate acyltransferase 1; pkn2, protein kinase N2.