Fig. 5.

σ₁-Receptor is expressed in rat mesenteric lymphatics. A: rat mesenteric lymphatics were harvested (10 lymphatic vessels per rat), and total RNA (50 ng) from mesenteric lymphatic vessels (3 samples from 3 different rats) was reverse transcribed into cDNA and RT-PCR was performed with specific primer/probe (FAM) sets for rat SIGMAR1 (NM_030996.1) and rat GAPDH (NM_017008.4). Two cDNA samples were also run with no primers as controls. B: protein lysates (6 samples from 6 rats, only 3 are shown) were prepared for SDS-PAGE and Western blot analysis for the detection of σ₁-receptor expression. With use of a rabbit anti-σ₁-receptor antibody (Invitrogen) at 0.25 µg/ml (specific for the mouse and rat but not human) and donkey anti-rabbit horseradish peroxidase-conjugated secondary antibody at 1:10,000, a single band was detected at ∼25 kDa in rat lymphatic (30 µg) and rat brain (10 µg) lysates.