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Green fluorescent protein (GFP) expression in the subfornical organ (SFO) and surrounding area (top) and in the hypothalamic paraventricular nucleus (PVN; bottom) 1 wk after SFO microinjection of vehicle (Veh; left) or tumor necrosis factor-α (TNF-α) receptor 1 (TNFR1) shRNA carrying GFP (right). In rats that received SFO microinjection of TNFR1 shRNA, GFP expression was observed in the SFO, with little expression in the surrounding tissue, but not in the PVN. Scale bar = 100 µm.
