Fig. 5.

NF-kB activity in the subfornical organ (SFO) and hypothalamic paraventricular nucleus (PVN) in rats 4 wk after coronary artery ligation to induce heart failure (HF) or sham operation (Sham) and 5 wk after SFO microinjection of tumor necrosis factor-α (TNF-α) receptor 1 (TNFR1) shRNA, scrambled (Con) shRNA, or vehicle (Veh). A: representative Western blots. B: phosphorylatled (p-)NF-κB p65 protein. C: IκB-α protein. Values are expressed as means ± SE and were corrected by β-actin; n = 7 for each group. Data were analyzed with two-way ANOVA followed by multiple-comparison tests [B, SFO, HF effect: F(1, 36) = 149, P < 0.0001; treatment effect: F(2, 36) = 7.02, P = 0.003. PVN, HF effect: F(1, 36) = 231, P < 0.0001; treatment effect: F(2, 36) = 12, P = 0.0001. C, SFO, HF effect: F(1, 36) = 86, P < 0.0001; treatment effect: F(2, 36) = 4.19, P = 0.02. PVN, HF effect: F(1, 36) = 112, P < 0.0001; treatment effect: F(2, 36) = 4.06, P = 0.03]. *P < 0.05 vs. Sham + Veh; †P < 0.05, HF + TNFR1 shRNA vs. HF + Veh.