Comparison of light absorption at 500 nm and 525 nm (the excitation and emission wavelengths used for fluorescein) of catalase (A), methemoglobin (B), TBAP (C), hemin (D), and albumin (E) (left) to the quenching effect of these compounds had on 3 μM fluorescein fluorescence (right). All compounds except albumin exhibited a concentration-dependent increase in absorption at both wavelengths that correlated with their inhibition of fluorescein fluorescence. All values are mean + SE, n = 4.