Table 1.
Sequences of the primers used for real-time RT-PCR
| Name | Sequence (5′-3′) | Product Length, bp | Accession Number |
|---|---|---|---|
| CCK1R | Forward: CCACCTACTTCATGGGCACT | 290 | NM_012688.3 |
| Reverse: CAGGAATGTTTGCCAGGACT | |||
| CCL2 | Forward: CCACCTACTTCATGGGCACT | 167 | NM_031530.1 |
| Reverse: CAGGAATGTTTGCCAGGACT | |||
| CRF | Forward: TCTCTCTGGATCTCACCTTCCACC | 77 | X03036 |
| Reverse: AGCTTGCTGAGCTAACTGCTCTGC | |||
| GAPDH | Forward: GACAACTTTGGCATCGTGGA | 133 | NM_017008 |
| Reverse: ATGCAGGGATGATGTTCTGG | |||
| GHS-R1a | Forward: CTCATCGGGAGGAAGCTATG | 216 | U94321 |
| Reverse: CAGGTTGCAGTACTGGCTGA | |||
| GLP-1R | Forward: CGAGCACTGTCCGTCTTCAT | 164 | NM_012728.1 |
| Reverse: AACCAGTAGTAGTTGGCCGC | |||
| IL-10 | Forward: CCTGCTCTTACTGGCTGGAG | 161 | NM_012854.2 |
| Reverse: TGTCCAGCTGGTCCTTCTTT | |||
| IL-6 | Forward: CCGGAGAGGAGACTTCACAG | 134 | NM_012589.2 |
| Reverse: CAGAATTGCCATTGCACAAC | |||
| IRa | Forward: ATGCCACCAATCCTTCCGTT | 268 | NM_017071.2 |
| Reverse: CTGGCCGAGTCGTCATACTC | |||
| LepR | Forward: CTGGGTTTGCGTATGGAAGT | 217 | NM_012596.1 |
| Reverse: CCAGTCTCTTGCTCCTCACC | |||
| mTOR | Forward: CCACCTACTTCATGGGCACT | 264 | NM_019906.1 |
| Reverse: CAGGAATGTTTGCCAGGACT | |||
| POMC | Forward: TCCATAGACGTGTGGAGCTG | 174 | AF510391.1 |
| Reverse: GACGTACTTCCGGGGATTTT | |||
| TNF-α | Forward: TGCCTCAGCCTCTTCTCATT | 108 | X66539.1 |
| Reverse: CCCATTTGGGAACTTCTCCT | |||
| TNF-β | Forward: TGAGTGGCTGTCTTTTGACG | 146 | NM_021578.2 |
| Reverse: TGGGACTGATCCCATTGATT |
CCL2, C-C motif chemokine ligand 2; CRF, corticotropin-releasing factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GHS-R1a, growth hormone secretagogue receptor 1a; GLP-1, glucagon-like peptide receptor 1; IL, interleukin; IRa, insulin receptor A; LepR, leptin receptor; mTOR, mammalian target of rapamycin; POMC, proopiomelanocortin; TGF-β, transforming growth factor-β; TNF-α: tumor necrosis factor-α.