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. 2017 Aug 25;49(10):541–548. doi: 10.1152/physiolgenomics.00090.2016

Fig. 3.

Fig. 3.

Mesenchymal stem cell (MSC) treatment increased collagen I content in diabetic wounds. A: Western blot analysis of collagen I and III protein content and loading control GAPDH in nondiabetic (Non-Db, Db/+) (n = 4) and diabetic (Db, Db/Db) (n = 4) wounds 7 days after injury, with or without MSC treatment (top). Equal amounts of protein samples were loaded to each lanes: 2 for nondiabetic wounds treated with PBS, 2 for diabetic wounds treated with PBS, 2 for nondiabetic treated with MSC, 2 for diabetic wounds treated with MSC, together with 1 lane for positive control. The positive control was loaded with standardized either collagen I or collagen III protein. B: collagen content was calculated after normalizing with standardized collagen control (bottom) using Image J analysis. Results are presented as means ± SD; *P value <0.05 considered statistically significant by 1-way ANOVA statistical test. Comparison was performed between Db/Db wounds and Db/+ wounds. Diabetic wounds showed significantly higher collagen I and III protein level with treatment of MSC.