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. 2017 Aug 25;49(10):541–548. doi: 10.1152/physiolgenomics.00090.2016

Fig. 6.

Fig. 6.

MSCs upregulate miR-29b levels in diabetic wounds and fibroblasts. Real-time quantitative PCR analysis of miR-29b in Db/+ (n = 4) and Db/Db (n = 4) wounds 7 days after injury with or without MSC treatment (A) and in dermal fibroblasts with MSC or vehicle coculture (B). The expression of miR-29b was significantly lower in diabetic wounds or diabetic fibroblasts. Treatment with MSC significantly induced miR-29b expression in diabetic wounds or diabetic fibroblasts. Real-time quantitative PCR analysis for MMP-9 (C), TIMP1(D), and miR-29b (E) in normal dermal fibroblasts under 5 mM (low glucose) or 25 mM (high glucose) conditions. MMP-9 was induced while miR-29b was reduced by high glucose. Results are presented as means ± SD. *P value <0.05, **P value <0.01. Comparison was performed between Db/Db and Db/+, as well as Db/Db with and without MSC treatment by 1-way ANOVA statistic test with multiple comparisons.