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. 2015 Jun 25;2:172–178. doi: 10.1016/j.bbrep.2015.06.007

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — The transcription levels of daf-16, sbp-1, nhr-49, cyp-35C1 and cyp-35A5 in wild-type, fat-5(tm420), cyp-35A2(gk317) and fat-5(tm420);cyp-35A2(gk317). Genes involved in aging- and lipid-regulatory pathways were selected to evaluate, by qPCR, changes in expression associated with the fat-5 and/or cyp-35A2 genotypes. The expression of daf-16 (Panel A), sbp-1 (Panel B), nhr-49 (Panel C), cyp-35C1 (Panel D) and cyp-35A5 (Panel E) were quantified in all four strains and the transcription level within wild-type was set to 1. Each bar presents the average fold-change of the transcript (normalized to rla-1) determined from two independent experiments with at least three technical repeats in each condition±the standard error of the mean (SEM).