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. Author manuscript; available in PMC: 2018 Oct 3.
Published in final edited form as: Cell Metab. 2017 Sep 14;26(4):672–685.e4. doi: 10.1016/j.cmet.2017.08.019

Figure 1. EODF treatment increases energy expenditure through WAT beiging.

Figure 1

(A) Cumulative food intake. n=14–16 mice/group.

(B) Body weight. n=14–16 mice/group.

(C) Normalized mass of body depots. n=7–8 mice/group.

(D) Circadian rectal temperature on fed day. n=14–16 mice/group.

(E–F) Daily total energy expenditure (E) and respiratory exchange ratio (F) during one cycle of EODF. On day 1 (“Fasted”), the EODF mice were fasted while the AL mice fed a chow diet. On day 2 (“Fed”), both groups had ad libitum access to chow. Data marked as “1 cycle” show the average value of the two days (“Fasted” plus “Fed”). n=4 mice/group.

(G) Representative image for inguinal WAT of AL(left) and EODF (right) mouse. Scale bar: 5 mm.

(H) Ucp1 mRNA expression in inguinal WAT in the fed state. n=7–8 mice/group.

(I) Representative H&E (upper) and UCP1 (lower) staining of inguinal WAT sections. Scale bar: 50 μm.

Data are presented as mean ± SEM. Different lowercase letters indicate different statistical significance by two-tailed unpaired t-test, a, p < 0.05; b, p < 0.01; c, p < 0.005; and d, p < 0.001 versus AL.

See also Figure S1–S5.