Table 1.

Changes in the expression levels of several progenitor/differentiation markers in dre-gabra2 deficient fish.

gene	24 hpf	48 hpf
fgf8	1.42±0.10*	1.51±0.07**
neuroD	0.70±0.08*	0.49±0.10*
notch1a	0.55±0.03***	1.92±0.50 (n.s.)
pax2a	0.65±0.10*	0.74±0.10 (n.s.)
pax6a	0.92±0.08 (n.s.)	1.00±0.10 (n.s.)
shh a	0.84±0.10 (n.s.)	1.15±0.16 (n.s.)
wnt1	0.42±0.05**	1.25±0.34 (n.s.)

Results represent the relative gene expression fold-changes of several genes known to be part of signalling cascades that determine the development of the CNS in gabra2 morpholino-injected embryos of 24 hpf and 48 hpf. Data shown are normalised to dre-eef1a1l1. n.s. denotes p>0.05 (not significant);

^*denotes p<0.05;

^**denotes p<0.01;

^***denotes p<0.001 (unpaired Student t-test, n=4). 50 embryos were used for each treatment and for each time point; the experiment was performed in triplicates and repeated four times.