Table 1.

Translation, aminoacylation activities, and solubility of 20 aaRS expressed in the PURE systems.

	Solubility (%)a	Aminoacylation activity (nM equivalent)b	Translation (nM)b	Ratio of activity to translation
AlaRS	88	310	230	1.3
ArgRS	89	460	290	1.6
AsnRS	92	150	50	3.0
AspRS	91	250	480	0.5
CysRS	93	1800	1500	1.2
GlnRS	86	250	350	0.7
GluRS	93	1500	350	4.3
GlyRS α	70	690	8900	1.3c
GlyRS β	88		540
HisRS	90	3000	930	3.2
IleRS	93	310	87	3.6
LeuRS	98	770	150	5.1
LysRS	94	2700	860	3.1
MetRS	97	71	140	0.5
PheRS α	37	Not detected	210
PheRS β	68		350
ProRS	94	350	150	2.3
SerRS	95	240	71	3.4
ThrRS	84	54	23	2.3
TrpRS	82	1900	420	4.5
TyrRS	87	610	170	3.6
ValRS	95	96	23	4.1

^aQuantified from the data shown in Fig. 2A.

^bMeasured with the reduced aaRS concentrations shown in Table S1. The activities are shown as a unit of molar equivalent of purified aaRS protein.

^cCalculated based on the translation amount of the β-subunit of GlyRS (GlyRS β).