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. 2017 Oct 9;6(10):e387. doi: 10.1038/oncsis.2017.85

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rescuing CMA activity through LAMP2a overexpression leads to compromised EMT. (a–c) MCF-10A-NC and MCF-10A-6SA cells were infected with pMSCV or LAMP2a, respectively. (a) Left: representative images of KFERQ- PA-mCherry1 puncta number and distribution in each group. Nuclei are labeled with DAPI. Scale bar represents 20 μm. Right: quantitative analysis of the number of puncta per cell in >20 cells in at least eight different fields. **P<0.01 and ***P<0.001. (b) Immunoblots analysis of indicated proteins. (c) Migration ability were analyzed by Transwell assay. Representative images of migrated cells were shown left and quantitative analysis of the migrated cell number in five different fields were shown right. Scale bar represents 20 μm. ***P<0.001. (d–f) MCF-10A cells were stably infected with pMSCV or LAMP2a, respectively, and followed by incubation under hypoxia for EMT induction. (d) Left: representative images of KFERQ- PA-mCherry1 puncta number and distribution in each group. Nuclei are labeled with DAPI. Scale bar represents 20 μm. Right: quantitative analysis of the number of puncta per cell in >20 cells in at least eight different fields. **P<0.01 and ***P<0.001. (e) Immunoblots analysis of indicated proteins. (f) Migration ability was analyzed by Transwell assay. Representative images of migrated cells were shown left and quantitative analysis of the migrated cell number in five different fields was shown right. Scale bar represents 20 μm. **P<0.01 and ***P<0.001.