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. 2017 Oct 23;6(10):e390. doi: 10.1038/oncsis.2017.88

Figure 1.

Figure 1

Identification of stromal-dependent genes in human breast tumors. (a) Breast cancer cell lines SKBR3 and AU565 treated with CAF-11 CM or control medium for 48 h were stained for F-actin. Nuclei were visualized using DAPI staining in blue. Representative images, scale bars, 50 μm. (b) Heatmaps from RNAseq data of SKBR3 and AU565 cell lines. Genes were classified according to their expression levels in cells treated with CAF-CMs normalized by their expression in control cells. (c) Heatmap depicting expression level of genes modulated by CAF-secreted factors identified in vitro in function of human breast tumors stromal score; left panel: genes upregulated by CAF-secreted factors (median centering, n=1042); right panel: genes unaffected by CAF-secreted factors (median centering, n=1042). (d) Mean expression level of genes modulated by CAF-secreted factors identified in vitro, in human breast tumors as a function of the tumor stromal score. (****P<0.0001, two-tailed unpaired Student’s t-test. Error bars=s.e.m). (e) Linear regression curve presenting the relationship between the mean expression level of genes upregulated by CAF-secreted factors identified in vitro and the tumor stromal score. (f) Linear regression curve presenting the relationship between genes unaffected by CAF-secreted factors identified in vitro and the tumor stromal score.