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IMAC purification of AxeA and AxeB; (A) AxeA crude sample is represented on lane 1, the flow through and the wash fractions on lanes 2 and 3, respectively and the purified AxeA is on lane 4. (B) Purified AxeB; lane a, b, c and d indicate crude AxeB, flow through, wash and elution fractions, respectively. Lanes M on both gels represents protein ladders.
