Table1.
Cloning efficiencies of the various molar ratios of insert DNA fragments to vector DNA in the SLiCE and In-Fusion methods.
| Cloning method | Cloning efficiencya |
|
|---|---|---|
| PrxIIE (AT3G52960) | G6PDH1 (AT5G35790) | |
| 0.5 kbp | 1.6 kbp | |
| SLiCE | ||
| 1:1 | 14/16 (87.5%) | 16/16 (100%) |
| 3:1 | 14/16 (87.5%) | 16/16 (100%) |
| 6:1 | 15/16 (93.8%) | 16/16 (100%) |
| 10:1 | 16/16 (100%) | 16/16 (100%) |
| 50:1 | 15/16 (93.8%) | 13/16 (81.3%) |
| In-Fusion | ||
| 1:1 | 16/16 (100%) | 16/16 (100%) |
| 3:1 | 16/16 (100%) | 16/16 (100%) |
| 6:1 | 15/16 (93.8%) | 16/16 (100%) |
| 10:1 | 16/16 (100%) | 16/16 (100%) |
| 50:1 | 16/16 (100%) | 14/16 (87.5%) |
a
Cloning efficiencies for the insert DNA are represented as the number of clones with the confirmed correct insert length by colony-PCR/number of colonies subjected to colony-PCR.