Fig. 1.

Expression of AQP8. A: Western blotting of AQP8 in 3T3–L1 cell lysate and homogenates of adipose tissue in C57BL/6J and BALB/cCrSlc mice. The lane for 3T3–L1 cells was loaded with 5 µg of protein and those for adipose tissues with 60 µg. The molecular weight of the band pointed by the arrow was estimated to be approximately 28kDa. B: Western blots for the expression of AQP8 in lysates of 3T3–L1 cells and AQP8-overexpressed cells (OE) and tissue homogenates of liver in C57BL/6J mice. The amount of sample applied was 5 µg protein for the cell lysates and 25 µg protein for the liver. C: N-glycosidase treatment of AQP8 proteins in 3T3–L1 cell lysate. Aliquots (25 µg protein) of the cell lysate were digested with (+) and without (-) N-glycosidase F for 24, 48, or 72 h at 37 °C. The bands pointed by the arrow-head, which have molecular weights of 30–38 kDa, were markedly reduced after the digestion.