Mitochondrial respiration through different segments of the electron transport chain. Routine respiration (A), Leak respiration (B), which were obtained by inhibition of ATP synthesis with oligomycin A, and the maximum oxygen flux, which were obtained after adding mitochondrial uncoupler FCCP (C). Basal levels were obtained by inhibition of complex III with antimycin A. Data are expressed as the mean±S.D. of nine independent experiments. (**p<0.01; n.s., not significant).