Fig. 2.

Flv alleviates Px-induced ER stress-mediated apoptosis. (a–c) SK-N-SH cells were pre-treated with or without 10 μg/ml Flv for 12 h followed by a 1-μM Px treatment with or without 10 μg/ml Flv for 24 h. CHOP, caspase 4, and caspase 3 were detected by Western blotting. GAPDH was used as an internal control. ^⁎p<0.05, ^⁎⁎p<0.01 (one-way ANOVA followed by Tukey–Kramer test. (d and e) SK-N-SH cells were treated with 10 μg/ml Flv for 12 h and 24 h. Sig-1R was detected by Western blotting. GAPDH was used as an internal control. ^⁎p<0.05, ^⁎⁎p<0.01 (Student's t-test).