Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov;27(11):1816–1829. doi: 10.1101/gr.222935.117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Dukler et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 1. — Characterizing the dynamic transcriptional response to celastrol using PRO-seq. (A) PRO-seq was applied to K562 cells collected before celastrol treatment (untreated/0 min) and at 10, 20, 40, 60, and 160 min after celastrol treatment. Two biological replicates were performed for each time point. (B) Distribution of log expression ratios (treated vs. untreated) for each time point (rlog is a regularized log₂ estimate obtained from DESeq2). Only genes classified as differentially expressed (DE) throughout the time course are represented. Notice that most DE genes (FDR ≤ 0.01) are down-regulated upon celastrol treatment. (C) A UCSC Genome Browser display showing raw PRO-seq data for two differentially expressed genes, EGR1 and KDM3B. EGR1 is rapidly and strongly repressed (immediate decrease of ∼80%), whereas KDM3B is more gradually repressed, losing ∼50% of its expression by 160 min.