Extended Data Figure 8. 3D classification of NOMPC-nanodisc particles.

The flowchart of classification procedures with RELION is shown. After 1 round of 2D classification, 190,879 particles were subjected to 3D refinement, yielding a 3.7Å map (C4 imposed). Following 3D classification of these particles, 175,314 particles were selected and refined, yielding the final map at 3.55Å resolution (C4 imposed), which was used to build the ‘consensus model’. Additional classification of these 175,314 particles using a mask to exclude all regions outside the AR domain gave 3 major classes, which were subsequently refined to ∼3.8-4.0Å resolution.