Table 1. Summary of cryo-EM data collection and model refinement.
| Nanodisc | Amphipol | |
|---|---|---|
| Data collection/processing | ||
|
| ||
| Voltage (kV) | 300 | 300 |
|
| ||
| Magnification | 31,000 | 31,000 |
|
| ||
| Defocus range (μm) | -1.5 – -3.0 | -1.5 – -3.0 |
|
| ||
| Pixel size (Å) | 1.2156 | 1.2156 |
|
| ||
| Total electron dose (e-/Å2) | 54 | 54 |
|
| ||
| Exposure time (s) | 8 | 8 |
|
| ||
| Number of images | 1873 | 4135 |
|
| ||
| Number of frames/image | 40 | 40 |
|
| ||
| Initial particle number | 337,716 (autopick) 190,879 (2D select) | 546,733 (autopick) 121,389 (2D select) |
|
| ||
| Final particle number | 175,314 | 52,065 |
|
| ||
| Resolution (unmasked, Å) | 4.38 | 7.15 |
|
| ||
| Resolution (masked, Å) | 3.55 | 4.54 |
|
| ||
| Refinement | ||
|
| ||
| Number of atoms | 40,888 | |
|
| ||
| R.m.s. deviations | ||
|
| ||
| Bond lengths (Å) | 0.01 | |
| Bond angles (0) | 1.03 | |
|
| ||
| Ramachandran | ||
|
| ||
| Favored (%) | 90.60 | |
| Allowed (%) | 9.06 | |
| Outlier (%) | 0.33 | |
|
| ||
| Molprobity score | 1.93 | |
|
| ||
| EMRinger score | 1.88 | |