Figure 4.

Apoptosis analysis by acridine orange/ethidium bromide (×10). (a) Negative control group (normal cells); (b) Nucleus showed yellow–green fluorescence by acridine orange staining (early apoptotic cells) and also showed orange fluorescence by ethidium bromide (late apoptotic cells) Acacia catechu seed extract 25 μg/ml treatment; (c) Nucleus showed yellow–green flurescence by acridine orange staining (early apoptotic cells) and showed orange fluorescence by ethidium bromide (late apoptotic cells) Acacia catechu seed extract 50 μg/ml treatment. (d) Quantification of apoptotic cells. Values are expressed as mean ± standard error mean (n = 3). ***P < 0.001