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. 2017 Sep 21;13(4):125–140. doi: 10.1080/15476278.2017.1358336

FIGURE 4.

FIGURE 4.

RARγ Maintains K5+ Cells Through Cell Cycle Regulation. (A) E12.5 explants were cultured for 48 hours with or without RARγ inhibitor with the incorporation of EdU for the last two hours. Scale bar, 100 μm. (B) ICC for M-phase marker pHH3 shows a decrease in positive staining in the main duct of RARγ-inhibited glands as compared to control. Scale bar, 100 μm. (C) Quantitative immunocytochemistry indicates significantly decreased EdU staining in the main duct of RARγ-inhibited glands as compared to vehicle control, indicating decreased numbers of cells in S-phase. EdU+ area normalized to total main duct area. Veh inhib n = 23, γ inhib n = 9 explants. Statistical analysis completed using Student's two-tailed t-test. ***p ≤ 0.001. (D) Quantitative immunocytochemistry for pHH3 shows a significant decrease in pHH3 positively stained area in the main duct of RARγ-inhibited glands as compared to control, indicating significantly fewer cells in M-phase. pHH3+ area normalized to total main duct area. Veh inhib n = 22, γ inhib n = 15 explants. Statistical analysis completed using Student's two-tailed t-test. *p = 0.04. Taken together, these results suggest G1 mediated cell cycle arrest.