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. Author manuscript; available in PMC: 2017 Nov 3.
Published in final edited form as: Nat Struct Mol Biol. 2015 Dec 7;23(1):74–80. doi: 10.1038/nsmb.3134

Figure 5. Membrane interaction elements of VP5.

Figure 5

(a) The histidine cluster of VP5. The fully conserved Glu316 is also highlighted. (b) Membrane permeability as measured by calcein release from liposomes as a function of pH for WT, and single- (H384F), double- (H384-5F) and triple- (H384-6F) mutants of the histidine His384–386 cluster of VP5. Error bars represent s.d. of n = 3 technical repeats. (c) External (viewed along the arrow in Figure 3b and internal sides of the core β-meander motif of the anchoring domain, showing a cluster of 5 and 7 aromatic residues on the internal and external side respectively. The SNARE-like WHXL motif (W411-L414) located on the β7 strand is shown in blue.