Fig. 5. Fuzzy clustering in selected coronal sections. Normalised signal intensity for P, Fe, Cu and Zn distribution and resulting fuzzy clustering analysis was aligned with coronal sections from the ARA: bregma +4.27 mm (olfactory bulbs; A); –1.16 mm (thalamus; B); –3.58 mm (midbrain; C) and –6.36 mm (cerebellum; D). *4 clusters used for (a). Alignment of metal clusters with the brain structure hierarchical tree for the ARA for the 4 sections analysed could specifically identify nuclei classes, and in some cases, specific structures ((e) see Table 1).