Fig. 1.

Immunoprecipitation and co-immunoprecipitation studies of Slick and Slack channels. Immunoprecipitation (IP) using DDM-solubilized mouse fore- and midbrain membranes with anti-Slick or anti-Slack antibodies. Different materials were used in Western blots: mouse fore- and midbrain synaptic plasma membranes (input), unsolubilized protein fraction, solubilized protein faction, unbound material (flow-through) and 2.5% of eluted protein fraction (eluate). Slack protein is co-purified by anti-Slick-antibody and vice versa.