Table 1.
Calorimetric parameters of viral unfolding recorded under different experimental conditions and in the presence of different monoclonal antibodies. Data are of the BPL-inactivated virus, except row one showing parameters of the non-inactivated virus.
| Unfolding parameters of rabies virus under different conditions | |||||
|---|---|---|---|---|---|
| Condition | Tm1 (°C) | ΔHcal1 (kcal/mol) | Tm2 (°C) | ΔHcal2 (kcal/mol) | |
| PBS, pH 7.4a | 62.13±0.1 | 455 | 73.04±0.1 | 434 | |
| PBS, pH 7.4b | 61.18±0.1 | 388 | 71.44±0.1 | 447 | |
| PBS, pH 7.3b | 61.40±0.1 | 313 | 71.63±0.1 | 368 | |
| PBS, pH 6.3b | 59.64±0.1 | 365 | 72.43±0.1 | 413 | |
| PBS, pH 5.8b | 62.24±0.1 | 352 | 71.91±0.1 | 362 | |
| PBS, pH 7.4, 5 mM DTTb | Broad transition | 71.78±0.1 | 444 | ||
| PBS, pH 7.4, after bromelain treatmentb | No unfolding event | 71.80±0.1 | 595 | ||
| Tm values determined for equimolar complexes of virusb with different monoclonal antibodies | |||||
| Antibody alone | Tm1 (°C) | Tm2 (°C) | Tm3 (°C) | ||
| D1-25 | 74.90±0.1 | 79.96±0.1 | – | ||
| 50AD1 | 58.88±1.5 | 67.36±0.1 | 75.95±0.1 | ||
| TJU11-12 | 76.28±0.1 | 80.05±0.1 | − | ||
| Virus alone | Tm1 (°C) | Tm2 (°C) | Tm3 (°C) | ||
| PBS, pH 7.4b | 61.18±0.5 | 71.44±0.1 | − | ||
| Antibody/virus complex | Tm1 (°C) | Tm2 (°C) | Tm3 (°C) | ||
| D1-25 | 67.90±0.5 | 73.58±0.1 | 79.94±0.1 | ||
| 50AD1 | 65.35±0.4 | 70.12±0.1 | − | ||
| TJU11-12 | 67.43±0.2 | 73.00±0.1 | 79.72±0.1 | ||
a
Non-inactivated virus.
b
BPL-inactivated virus.