Fig 2. ZIKV infection of non-pregnant guinea pigs.

Groups of guinea pigs (N = 4) were infected with 1x10⁷ or 1X10⁸ PFU of ZIKV or sham injected. (A) The weight change of experimentally challenged guinea pigs over time was recorded. The mean weight change in each group of challenged animals and the standard error at each time point is plotted. (B) Viremia in whole blood from challenged guinea pigs was quantified using qRT-PCR. Solid symbols indicate animals challenged with 1X10⁷ PFU; open symbols indicate animals challenged with 1X10⁸ PFU. (C) Select plasma samples from guinea pigs challenged with 1X10⁸ PFU of ZIKV were examined using a ZIKV NS1 ELISA. The number of ZIKV genome copies/ml and the magnitude of antigenemia, as assessed in ng/ml NS1 in blood or plasma from each animal at the indicated time point post-infection are shown. ND: not detectable.