Fig 4. Anti-ZIKV antibody production by infected guinea pigs.
To assess whether an antibody response to ZIKV proteins is elicited in challenged guinea pigs, purified ZIKV Env and NS1 and semi-purified viral particles were resolved by SDS-PAGE, transferred to membranes, and immunoblotted. (A) Coomassie stained gel showing the sizes of input protein. (B-E) Proteins were transferred to membranes and immunoblotted using plasma collected from four ZIKV challenged pregnant guinea pigs either the day before viral challenge (D-1) or the day of delivery (Term).