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In vitro stabilization of NEU2 by siastatin B and DANA. Thermostability of 4-MU-NANA-degrading activity of purified human NEU2 after pre-incubation in 0.1 M sodium acetate buffer at pH 6.0 (●), 5.0 (■) or 4.0 (Δ) at 37 °C for different time intervals (A). Residual NEU2 activity after pre-incubation in 0.1 M sodium acetate buffer (pH 4.0) in the presence of siastatin B (B) and DANA (C) at different concentrations of 50 µM (Δ), 100 µM (■), 200 µM (●) or 0 (○) µM.
