Table 2.

Inhibitory effects of siastatin B and DANA toward recombinant human NEU2 as to the natural substrate α2,3-sialyllactose^a.

Sample	pH	Sialic acid		Relative rate of hydrolysis (%)
		Bound	Released
		µmol
NEU2	6.0	885	808	100
	4.0	867	769	100
NEU2/siastatin Bb	6.0	866	387	47.8
	4.0	890	277	36.1
NEU2/DANAb	6.0	855	336	41.6
	4.0	882	494	64.3

^aSubstrate was incubated for 1 h under the standard assay conditions.

^bSiastatin B and DANA: final concentration 600 µM.