Figure 2. Different classes of transcription factors interact and regulate cellular identity.

The top of the figure illustrates the initial steps of enhancer selection in closed chromatin consisting of regularly positioned nucleosomes. Green and blue shading represents closely spaced binding sites for LDTFs. Below, collaborative interactions between LDTFs generate primed enhancers, characterized by a nucleosome-free region and monomethylated H3K4 (H3K4me1). At the bottom of the figure, active SDTFs localize to primed enhancers, resulting in recruitment of coactivators and RNA polymerase II and generation of enhancer RNAs (eRNAs).