Figure 5. Colonic injury upregulates WISP-1 synthesis and secretion.

(A) WISP1 mRNA levels were determined in tissue obtained from punch biopsies of intact colon (IT) and wounded colonic mucosa on post-injury days 1, 2, and 3 (***P < 0.001 and *P < 0.05, n = 3, mean ± SEM). (B) Laser confocal micrographs of frozen sections from intact colon and wounds on days 1 and 3 after wounding show WISP-1 expression (green), F-actin (red), and nuclei (blue). Scale bar: 100 μm. Original magnification ×20. (C) WISP1 mRNA levels in colon biopsies from healthy controls and IBD patients with active inflammation in the colon (***P < 0.001, n = 5, mean ± SEM). (D) Laser confocal micrographs of frozen sections from healthy and active IBD tissue show WISP-1 expression (green), F-actin (red), and nuclei (blue). Scale bar: 100 μm. UC, ulcerative colitis; CD, Crohn’s disease. (E) Lysates of healthy and active IBD tissue from human colon samples were immunoblotted to detect WISP-1, IL-10Rα, GBP-1, and GAPDH. B, D, and E show representative images from 3 experiments. Sample information pertaining to patient diagnosis, age, and treatment is provided in Supplemental Table 1. All statistical comparisons were performed using ANOVA with Tukey’s multiple comparisons post test.